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| In November 1997, The Office for Protection from Research Risks (OPRR) at the National Institutes of Health, forwarded a letter to all Public Health Service (PHS) awardee institutions and Institutional Animal Care and Use Committees (IACUCs) on avoiding or minimizing discomfort, distress, and pain in the care and use of animals for the production of monoclonal antibodies using mouse ascites method since there is evidence that the ascites method of monoclonal antibody production causes discomfort, distress, or pain. As a result, the Immunology Core has developed a method to generate antibody using an in-vitro method that compare favorably (cost and quantity of antibody) to the ascities method. The method used by the Immunolgy Core utilizes gas-permeable tissue culture bags (standard scale - 1L) or roller bottles (small scale - 100mL) and a media that is especially formulated for antibody production. Typically, antibody concentrations are about 0.3mg/mL using this technique with variations between 0.1-1mg/mL. These concentatrations are about 5 to 10 fold what is commonly obtained using other methods.
For most hybridomas, it will take about 2 weeks to adapt the cell line to the media and 3 weeks to produce the high-yield final material. If requested, the Immunology Core can purify the antibodies from the raw material. FEES: |
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