Antibody Purification

• Protein G affinity chromatography
• Monoclonal or polyclonal antibodies
• Purified antibodies will be dialyzed into PBS or a buffer of choice (if requested)
• Standard scale (5mL Protein G column) - yield: ~10-20mg antibody
• Small scale (1mL Protein G column) - yield: ~1-3mg antibody

FEES:
      $562 for standard scale (5mL Protein G column)
      $214 for small scale (1mL Protein G column)

NOTE: We can also affinity-purify rabbit polyclonal sera using the specific peptide antigen. Contact the Immunology Core for more details and pricing.

Antibody Conjugation

The Immunology Core Facility can conjugate your purified monoclonal or polyclonal antibody to any of the following: (Call for pricing)

• Alkaline Phosphatase (AP)
• Horseradish Peroxidase (HRP)
• Fluorescein (FITC)
• Phycoerythrin (PE)
• Cy5PE
• Cy7PE
• Texas Red (TR)
• Allophycocyanin (APC)
• Cy5
• Cy7APC
• Cascade Blue
• Biotin

UNC Immunology Core Facility
CB# 7182 / 444 Taylor Hall, Chapel Hill, NC 27599

Copyright © 2003 UNC-CH Immunology Core Facility. All rights reserved.